Systemic Bacteriology · NEET PG Preparation Microbiology
A 22-year-old male presents with acute urethritis characterized by a purulent urethral discharge. Gram stain of the exudate reveals intracellular gra…
Practice question from Systemic Bacteriology (NEET PG Preparation Microbiology), with the correct answer and a step-by-step solution.
medium · MCQ · Q-1AF8F8
A 22-year-old male presents with acute urethritis characterized by a purulent urethral discharge. Gram stain of the exudate reveals intracellular gram-negative diplococci within neutrophils. The organism requires specialized media containing hemin (Factor X) and NAD (Factor V) to grow, or can be cultured on Chocolate agar enriched with these factors. Wait, re-evaluating the organism causing purulent urethritis with gram-negative intracellular diplococci: what is the classic diagnostic growth requirement for Neisseria gonorrhoeae vs Haemophilus influenzae? Which growth medium is selectively used to isolate Neisseria gonorrhoeae from clinical specimens?
- A.Thayer-Martin mediumCorrect answer
- B.Lowenstein-Jensen medium
- C.TCBS medium
- D.Buffered Charcoal Yeast Extract (BCYE) agar
Solution
Neisseria gonorrhoeae is a gram-negative diplococcus causing gonorrhea. Because clinical specimens often contain normal flora, selective media such as Thayer-Martin medium (which contains antibiotics like vancomycin, colistin, nystatin, and trimethoprim to inhibit normal flora) is used for isolation. LJ medium is for mycobacteria, TCBS for Vibrio cholerae, and BCYE for Legionella.
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