Biotechnology: Principles and Processes · Class 12 · CBSE · Science Biology
A restriction enzyme recognises the sequence 5'-GAATTC-3' and cuts between G and A on both DNA strands. What type of ends are produced?
Practice question from Biotechnology: Principles and Processes (Class 12 · CBSE · Science Biology), with the correct answer and a step-by-step solution.
easy · MCQ · Q-71DED8
A restriction enzyme recognises the sequence 5'-GAATTC-3' and cuts between G and A on both DNA strands. What type of ends are produced?
- A.Blunt ends with no single-stranded extension
- B.Sticky ends with a 5' single-stranded AATT overhangCorrect answer
- C.Sticky ends with a 3' single-stranded AATT overhang
- D.RNA-DNA hybrid ends
Solution
The staggered cut made by this restriction enzyme produces complementary sticky ends. The exposed sequence is AATT at the 5' end of each fragment.
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