Biotechnology: Principles and Processes · Class 12 · CBSE · Science Biology
During bacterial transformation, treatment of cells with calcium chloride followed by brief heat shock mainly helps to:
Practice question from Biotechnology: Principles and Processes (Class 12 · CBSE · Science Biology), with the correct answer and a step-by-step solution.
easy · MCQ · Q-98216E
During bacterial transformation, treatment of cells with calcium chloride followed by brief heat shock mainly helps to:
- A.Cut the plasmid DNA at specific recognition sites
- B.Increase the uptake of foreign DNA by bacterial cellsCorrect answer
- C.Join the foreign DNA to the bacterial chromosome
- D.Replicate the plasmid outside the bacterial cell
Solution
Calcium chloride treatment and heat shock make bacterial cells competent by increasing the efficiency with which they take up foreign DNA, such as recombinant plasmids.
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