Cheat sheet · Class 12 · CBSE · Science Biology

Biotechnology and its Applications cheat sheet

The whole chapter on one page: key formulas, facts and the points to remember, for quick revision before a test.

CBSE Class 12 · Biology

Cheat sheet

Chapter 10Biotechnology and its Applications

Bt cotton, RNAi tobacco, insulin, ADA gene therapy — biotech's highlights.

1. Agriculture

  • Bt cotton: cry genes (cryIAc, cryIIAb) make Bt toxin against bollworms.
  • RNA interference: protects tobacco from nematode Meloidogyne.
  • GM crops: pest resistance, less chemical use.

2. Medicine

  • Human insulin (Eli Lilly, 1983): A and B chains made separately and joined.
  • Gene therapy: ADA deficiency (first in 1990).
  • Molecular diagnosis: PCR, ELISA, probes.

3. Transgenic Animals & Ethics

  • Rosie the cow: human α-lactalbumin-rich milk.
  • Used for testing vaccines and chemical safety.
  • GEAC regulates GM research in India.
  • Biopiracy concerns (basmati, turmeric patents).

4. Key Points

  • Bt toxin is activated in the insect gut (alkaline).
  • Golden rice: vitamin A.
  • Pro-insulin has an extra C-peptide.
  • Patents protect biotech inventions.

Remember: Bt cotton | RNAi | insulin | gene therapy | GEAC

Biotechnology and its Applications: questions and notes

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